Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society - l'unam - université nantes angers le mans Accéder directement au contenu
Article Dans Une Revue (Article De Synthèse) Frontiers in Endocrinology Année : 2020

Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society

Josefine Tratwal
  • Fonction : Auteur
Rossella Labella
  • Fonction : Auteur
Nathalie Bravenboer
  • Fonction : Auteur
Eleni Douni
  • Fonction : Auteur
Erica Scheller
  • Fonction : Auteur
Dimitrios Karampinos
  • Fonction : Auteur
Biagio Palmisano
  • Fonction : Auteur
Antonella Poloni
  • Fonction : Auteur
Maria Moreno-Aliaga
  • Fonction : Auteur
Jackie Fretz
  • Fonction : Auteur
Matthew Rodeheffer
  • Fonction : Auteur
Parastoo Boroumand
  • Fonction : Auteur
Mark Horowitz
  • Fonction : Auteur
Bram van Der Eerden
  • Fonction : Auteur
Annegreet Veldhuis-Vlug
  • Fonction : Auteur
Olaia Naveiras
  • Fonction : Auteur

Résumé

The interest in bone marrow adiposity (BMA) has increased over the last decade due to its association with, and potential role, in a range of diseases (osteoporosis, diabetes, anorexia, cancer) as well as treatments (corticosteroid, radiation, chemotherapy, thiazolidinediones). However, to advance the field of BMA research, standardization of methods is desirable to increase comparability of study outcomes and foster collaboration. Therefore, at the 2017 annual BMA meeting, the International Bone Marrow Adiposity Society (BMAS) founded a working group to evaluate methodologies in BMA research. All BMAS members could volunteer to participate. The working group members, who are all active preclinical or clinical BMA researchers, searched the literature for articles investigating BMA and discussed the results during personal and telephone conferences. According to the consensus opinion, both based on the review of the literature and on expert opinion, we describe existing methodologies and discuss the challenges and future directions for (1) histomorphometry of bone marrow adipocytes, (2) ex vivo BMA imaging, (3) in vivo BMA imaging, (4) cell isolation, culture, differentiation and in vitro modulation of primary bone marrow adipocytes and bone marrow stromal cell precursors, (5) lineage tracing and in vivo BMA modulation, and (6) BMA biobanking. We identify as accepted standards in BMA research: manual histomorphometry and osmium tetroxide 3D contrat-enhanced μCT for ex vivo quantification, specific MRI sequences (WFI and H-MRS) for in vivo studies, and RT-qPCR with a minimal four gene panel or lipid-based assays for in vitro quantification of bone marrow adipogenesis. Emerging techniques are described which may soon come to complement or substitute these gold standards. Known confounding factors and minimal reporting standards are presented, and their use is encouraged to facilitate comparison across studies. In conclusion, specific BMA methodologies have been developed. However, important challenges remain. In particular, we advocate for the harmonization of methodologies, the precise reporting of known confounding factors, and the identification of methods to modulate BMA independently from other tissues. Wider use of existing animal models with impaired BMA production (e.g., Pfrt$^−$$^/$$^−$, Kit$^W$$^/$$^W$$^-$$^V$) and development of specific BMA deletion models would be highly desirable for this purpose.
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Dates et versions

hal-02496659 , version 1 (07-05-2024)

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Josefine Tratwal, Rossella Labella, Nathalie Bravenboer, Greet Kerckhofs, Eleni Douni, et al.. Reporting Guidelines, Review of Methodological Standards, and Challenges Toward Harmonization in Bone Marrow Adiposity Research. Report of the Methodologies Working Group of the International Bone Marrow Adiposity Society. Frontiers in Endocrinology, 2020, 11, pp.65. ⟨10.3389/fendo.2020.00065⟩. ⟨hal-02496659⟩
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