A unifying model for the propagation of prion proteins in yeast brings insight into the [PSI+] prion - Institut Camille Jordan Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2020

A unifying model for the propagation of prion proteins in yeast brings insight into the [PSI+] prion

Résumé

The use of yeast systems to study the propagation of prions and amyloids has emerged as a crucial aspect of the global endeavor to understand those mechanisms. Yeast prion systems are intrinsically multi-scale: the molecular chemical processes are indeed coupled to the cellular processes of cell growth and division to influence phenotypical traits, observable at the scale of colonies. We introduce a novel modeling framework to tackle this difficulty using impulsive differential equations. We apply this approach to the [PSI+] yeast prion, which associated with the misconformation and aggregation of Sup35. We build a model that reproduces and unifies previously conflicting experimental observations on [PSI+] and thus sheds light onto characteristics of the intracellular molecular processes driving aggregate replication. In particular our model uncovers a kinetic barrier for aggregate replication at low densities, meaning the change between prion or prion-free phenotype is a bi-stable transition. This result is based on the study of prion curing experiments, as well as the phenomenon of colony sectoring, a phenotype which is often ignored in experimental assays and has never been modeled. Furthermore, our results provide further insight into the effect of guanidine hydrochloride (GdnHCl) on Sup35 aggregates. To qualitatively reproduce the GdnHCl curing experiment, aggregate replication must not be completely inhibited, which suggests the existence of a mechanism different than Hsp104-mediated fragmentation. Those results are promising for further development of the [PSI+] model, but also for extending the use of this novel framework to other yeast prion or amyloid systems.
Fichier principal
Vignette du fichier
2020.01.13.904060v1.full.pdf (1.34 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02501966 , version 1 (08-03-2020)

Identifiants

Citer

Paul Lemarre, Suzanne S Sindi, Laurent Pujo-Menjouet. A unifying model for the propagation of prion proteins in yeast brings insight into the [PSI+] prion. 2020. ⟨hal-02501966⟩
52 Consultations
44 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More